H2N2V1, Main, Exploration, bibRecord, 002090

Establishing a genetic recombination map for murine coronavirus strain A59 complementation groups

Identifieur interne : 002090 ( Main/Exploration ); précédent : 002089; suivant : 002091

Establishing a genetic recombination map for murine coronavirus strain A59 complementation groups

Auteurs : Ralph S. Baric [États-Unis] ; Kaisong Fu [États-Unis] ; Mary C. Schaad [États-Unis] ; Stephen A. Stohlman [États-Unis]

Source :

Virology [ 0042-6822 ] ; 1990.

RBID : ISTEX:DF3279F593A64C78045CF0A68AB01A45AFFBE616

English descriptors

Teeft :
- Animal viruses, Apthovirus, Baric, Base pairs, Cistron, Complementation, Complementation group, Complementation groups, Coronavirus, Coronaviruses, Crossover, Crossover events, Different complementation groups, Genetic, Genetic functions, Genetic recombination, Genome, Glycoprotein, Glycoprotein gene, High frequency, Input multiplicity, Keck, Late event, Mackenzie, Makino, Mouse hepatitis virus, Mrna, Murine, Murine coronavirus, Mutant, Negative strand, Nonpermissive temperature, Nonstructural, Nonstructural proteins, Nucleotide, Nucleotide domains, Phenotype, Poliovirus, Polymerase, Polymerase region, Recombinant, Recombinant virus, Recombinant viruses, Recombination, Recombination events, Recombination frequencies, Recombination frequency, Recombination techniques, Restrictive temperature, Reversion, Reversion frequencies, Same complementation group, Sawicki, Schaad, Siddell, Sindbis virus, Spaan, Stohlman, Subgenomic, Subgenomic mrnas, Transcription, Viral, Virology, Virus, Virus growth cycle.

Abstract

Abstract: MHV-A59 temperature-sensitive mutants, representing one RNA+ and five RNA− complementation groups, were isolated and characterized by genetic recombination techniques. Maximum recombination frequencies occurred under multiplicities of infection greater than 10 each in which 99.99% of the cells were co-infected. Recombination frequencies between different is mutants increased steadily during infection and peaked late in the virus growth cycle. These data suggest that recombination is a late event in the virus replication cycle. Recombination frequencies were also found to range from 63 to 20,000 times higher than the sum of the spontaneous reversion frequencies of each is mutant used in the cross. Utilizing standard genetic recombination techniques, the five RNA− complementation groups of MHV-A59 were arranged into an additive, linear, genetic map located at the 5′ end of the genome in the 23-kb polymerase region. These data indicate that at least five distinct functions are encoded in the MHV polymerase region which function in virus transcription. Moreover, using well-characterized is mutants the recombination frequency for the entire 32-kb MHV genome was found to approach 25% or more. This is the highest recombination frequency described for a nonsegmented, linear, plus-polarity RNA virus.

Url:

https://api.istex.fr/ark:/67375/6H6-8QV6HPPR-R/fulltext.pdf

DOI: 10.1016/0042-6822(90)90530-5

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000F77
to stream Istex, to step Curation: 000F77
to stream Istex, to step Checkpoint: 000E20
to stream Main, to step Merge: 002191
to stream Main, to step Curation: 002090

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Establishing a genetic recombination map for murine coronavirus strain A59 complementation groups</title>
<author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name>
</author>
<author><name sortKey="Fu, Kaisong" sort="Fu, Kaisong" uniqKey="Fu K" first="Kaisong" last="Fu">Kaisong Fu</name>
</author>
<author><name sortKey="Schaad, Mary C" sort="Schaad, Mary C" uniqKey="Schaad M" first="Mary C." last="Schaad">Mary C. Schaad</name>
</author>
<author><name sortKey="Stohlman, Stephen A" sort="Stohlman, Stephen A" uniqKey="Stohlman S" first="Stephen A." last="Stohlman">Stephen A. Stohlman</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:DF3279F593A64C78045CF0A68AB01A45AFFBE616</idno>
<date when="1990" year="1990">1990</date>
<idno type="doi">10.1016/0042-6822(90)90530-5</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-8QV6HPPR-R/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000F77</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F77</idno>
<idno type="wicri:Area/Istex/Curation">000F77</idno>
<idno type="wicri:Area/Istex/Checkpoint">000E20</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000E20</idno>
<idno type="wicri:doubleKey">0042-6822:1990:Baric R:establishing:a:genetic</idno>
<idno type="wicri:Area/Main/Merge">002191</idno>
<idno type="wicri:Area/Main/Curation">002090</idno>
<idno type="wicri:Area/Main/Exploration">002090</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Establishing a genetic recombination map for murine coronavirus strain A59 complementation groups</title>
<author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Parasitology and Lab Practice, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7400</wicri:regionArea>
<orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName>
<placeName><settlement type="city">Chapel Hill (Caroline du Nord)</settlement>
<region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fu, Kaisong" sort="Fu, Kaisong" uniqKey="Fu K" first="Kaisong" last="Fu">Kaisong Fu</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Parasitology and Lab Practice, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7400</wicri:regionArea>
<orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName>
<placeName><settlement type="city">Chapel Hill (Caroline du Nord)</settlement>
<region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Schaad, Mary C" sort="Schaad, Mary C" uniqKey="Schaad M" first="Mary C." last="Schaad">Mary C. Schaad</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Parasitology and Lab Practice, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7400</wicri:regionArea>
<orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName>
<placeName><settlement type="city">Chapel Hill (Caroline du Nord)</settlement>
<region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Stohlman, Stephen A" sort="Stohlman, Stephen A" uniqKey="Stohlman S" first="Stephen A." last="Stohlman">Stephen A. Stohlman</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Neurology and Microbiology, University of Southern California, School of Medicine, 2025 Zonal Avenue, Los Angeles, California 90007</wicri:regionArea>
<orgName type="university">Université de Californie du Sud</orgName>
<placeName><settlement type="city">Los Angeles</settlement>
<region type="state">Californie</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virology</title>
<title level="j" type="abbrev">YVIRO</title>
<idno type="ISSN">0042-6822</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1990">1990</date>
<biblScope unit="volume">177</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="646">646</biblScope>
<biblScope unit="page" to="656">656</biblScope>
</imprint>
<idno type="ISSN">0042-6822</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0042-6822</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Animal viruses</term>
<term>Apthovirus</term>
<term>Baric</term>
<term>Base pairs</term>
<term>Cistron</term>
<term>Complementation</term>
<term>Complementation group</term>
<term>Complementation groups</term>
<term>Coronavirus</term>
<term>Coronaviruses</term>
<term>Crossover</term>
<term>Crossover events</term>
<term>Different complementation groups</term>
<term>Genetic</term>
<term>Genetic functions</term>
<term>Genetic recombination</term>
<term>Genome</term>
<term>Glycoprotein</term>
<term>Glycoprotein gene</term>
<term>High frequency</term>
<term>Input multiplicity</term>
<term>Keck</term>
<term>Late event</term>
<term>Mackenzie</term>
<term>Makino</term>
<term>Mouse hepatitis virus</term>
<term>Mrna</term>
<term>Murine</term>
<term>Murine coronavirus</term>
<term>Mutant</term>
<term>Negative strand</term>
<term>Nonpermissive temperature</term>
<term>Nonstructural</term>
<term>Nonstructural proteins</term>
<term>Nucleotide</term>
<term>Nucleotide domains</term>
<term>Phenotype</term>
<term>Poliovirus</term>
<term>Polymerase</term>
<term>Polymerase region</term>
<term>Recombinant</term>
<term>Recombinant virus</term>
<term>Recombinant viruses</term>
<term>Recombination</term>
<term>Recombination events</term>
<term>Recombination frequencies</term>
<term>Recombination frequency</term>
<term>Recombination techniques</term>
<term>Restrictive temperature</term>
<term>Reversion</term>
<term>Reversion frequencies</term>
<term>Same complementation group</term>
<term>Sawicki</term>
<term>Schaad</term>
<term>Siddell</term>
<term>Sindbis virus</term>
<term>Spaan</term>
<term>Stohlman</term>
<term>Subgenomic</term>
<term>Subgenomic mrnas</term>
<term>Transcription</term>
<term>Viral</term>
<term>Virology</term>
<term>Virus</term>
<term>Virus growth cycle</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: MHV-A59 temperature-sensitive mutants, representing one RNA+ and five RNA− complementation groups, were isolated and characterized by genetic recombination techniques. Maximum recombination frequencies occurred under multiplicities of infection greater than 10 each in which 99.99% of the cells were co-infected. Recombination frequencies between different is mutants increased steadily during infection and peaked late in the virus growth cycle. These data suggest that recombination is a late event in the virus replication cycle. Recombination frequencies were also found to range from 63 to 20,000 times higher than the sum of the spontaneous reversion frequencies of each is mutant used in the cross. Utilizing standard genetic recombination techniques, the five RNA− complementation groups of MHV-A59 were arranged into an additive, linear, genetic map located at the 5′ end of the genome in the 23-kb polymerase region. These data indicate that at least five distinct functions are encoded in the MHV polymerase region which function in virus transcription. Moreover, using well-characterized is mutants the recombination frequency for the entire 32-kb MHV genome was found to approach 25% or more. This is the highest recombination frequency described for a nonsegmented, linear, plus-polarity RNA virus.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Californie</li>
<li>Caroline du Nord</li>
</region>
<settlement><li>Chapel Hill (Caroline du Nord)</li>
<li>Los Angeles</li>
</settlement>
<orgName><li>Université de Californie du Sud</li>
<li>Université de Caroline du Nord à Chapel Hill</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name>
</region>
<name sortKey="Fu, Kaisong" sort="Fu, Kaisong" uniqKey="Fu K" first="Kaisong" last="Fu">Kaisong Fu</name>
<name sortKey="Schaad, Mary C" sort="Schaad, Mary C" uniqKey="Schaad M" first="Mary C." last="Schaad">Mary C. Schaad</name>
<name sortKey="Stohlman, Stephen A" sort="Stohlman, Stephen A" uniqKey="Stohlman S" first="Stephen A." last="Stohlman">Stephen A. Stohlman</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002090 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002090 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:DF3279F593A64C78045CF0A68AB01A45AFFBE616
   |texte=   Establishing a genetic recombination map for murine coronavirus strain A59 complementation groups
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

	Serveur d'exploration H2N2
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration H2N2

Establishing a genetic recombination map for murine coronavirus strain A59 complementation groups

Establishing a genetic recombination map for murine coronavirus strain A59 complementation groups

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri